Preparation and Purification of Fullerene C60

The preparation and purification of fullerene C60 involves a series of steps to synthesize, isolate, and refine the compound. Here’s an outline of the process:

Preparation of Fullerene C60

The most common method to prepare fullerene C60 is through the arc-discharge method:

Arc-Discharge Method

A.Materials:

    • Graphite rods (high-purity)
    • Helium or argon gas (inert atmosphere)

    B.Process:

    1.Generation of Carbon Plasma:

      • A direct current arc is struck between two graphite electrodes in an inert gas atmosphere.
      • The high temperature (around 3000–4000 K) vaporizes the graphite, producing a carbon plasma.

      2.Formation of Fullerenes:

        • As the carbon vapor cools rapidly, fullerene molecules such as fullerene C60 and C70 form in the soot.
        Preparation and Purification of Fullerene C60-Xi'an Lyphar Biotech Co., Ltd

        Alternative Synthesis Methods

        A.Laser Ablation:

          • A high-power laser is used to vaporize graphite in an inert gas atmosphere.

          B.Combustion Method:

            • Hydrocarbon fuels (e.g., benzene) are burned under oxygen-deficient conditions, producing fullerene-rich soot.

            Extraction and Purification

            Once the soot containing fullerene C60 is produced, the next steps are to extract and purify the fullerene.

            A.Extraction

              Solvent Extraction:

              • The soot is treated with an organic solvent, such as toluene, benzene, or carbon disulfide (CS2).
              • Fullerenes dissolve, leaving behind insoluble impurities like amorphous carbon.

              B.Purification

                Chromatographic Techniques:

                1.Column Chromatography:

                  • The fullerene-containing solution is passed through a column filled with a stationary phase, such as silica gel or alumina.
                  • fullerene C60 and fullerene C70 are separated based on their polarity and molecular weight.
                  • Typically, fullerene C60 elutes faster than fullerene C70 .
                  Preparation and Purification of Fullerene C60-Xi'an Lyphar Biotech Co., Ltd

                  2.High-Performance Liquid Chromatography (HPLC):

                    • This method offers higher resolution and efficiency.
                    • It uses a nonpolar stationary phase and an organic solvent as the mobile phase to separate fullerene C60.

                    3.Recrystallization:

                      • Further purification can be achieved by recrystallizing fullerene C60 from solvents like toluene or benzene, yielding high-purity crystals.

                      Characterization of Purified Fullerene C60

                      To confirm the purity and structure of fullerene C60 , the following methods are used:

                      A.Spectroscopy:

                        • UV-Vis: Shows characteristic absorption peaks for fullerene C60 .
                        • Infrared (IR): Detects specific vibrations of the fullerene molecule.
                        • Nuclear Magnetic Resonance (NMR): Confirms the symmetry and structure.

                        B.Mass Spectrometry:

                          • Confirms the molecular weight of fullerene C60 (720 g/mol).

                          C.X-Ray Diffraction (XRD):

                            • Identifies crystalline structures of the purified fullerene.
                            Preparation and Purification of Fullerene C60-Xi'an Lyphar Biotech Co., Ltd

                            End Product

                            After purification, fullerene C60 appears as a dark purple or black crystalline material with high purity, ready for applications in materials science, electronics, or medicinal research.